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Association of miRNA-122-binding site polymorphism at the interleukin-1 α gene and its interaction with

null

《医学前沿（英文）》 2014年第8卷第2期页码 217-226 doi: 10.1007/s11684-014-0326-2

摘要：

This study was designed to investigate the contribution of miRNA-122-binding site polymorphism at the IL-1A gene and its multiplicative interactions with hepatitis B virus (HBV) mutations in the risk of hepatocellular carcinoma (HCC). A total of 1021 healthy controls, 302 HBV surface antigen (HBsAg) seroclearance subjects, and 2011 HBsAg-positive subjects (including 1021 HCC patients) were enrolled in this study. Quantitative PCR was used to genotype rs3783553. HBV mutations were determined by direct sequencing. Multivariate logistic regression analyses were performed to test the associations of rs3783553, mutations, and their interactions with the risk of HCC. No significant association was found between rs3783553 and the risk of HCC among healthy controls, HBsAg seroclearance subjects, HBsAg-positive subjects without HCC, and all controls. Additionally, rs3783553 was not significantly associated with chronic HBV infection, liver cirrhosis, HBV e antigen seroconversion, abnormal alanine aminotransferase, and high viral load (>10⁴ copies/ml). However, the TTCA insertion allele of rs3783553 was significantly associated with an increased frequency of HBV C7A mutation compared with homozygous TTCA deletion carriers [(del/ins+ ins/ins) vs. del/del, adjusted odds ratio (OR)=1.48, 95% confidence interval (CI)=1.09-2.02, P=0.013]. Multiplicative interaction of rs3783553 with HBV preS deletion significantly reduced the risk of HCC in males, with an adjusted OR of 0.64 (95% CI=0.42-0.98; P=0.041) after age and HBV genotype were adjusted. Although rs3783553 did not significantly affect genetic susceptibility to HBV-related HCC, its variant allele may predispose the host to selecting HBV C7A mutation during evolution and significantly reduce the risk of HCC caused by HBV preS deletion. This study provides an insight into the complex host-virus interaction in HBV-induced hepatocarcinogenesis and is helpful in determining HBsAg-positive subjects who are likely to develop HCC.

关键词： hepatocellular carcinoma (HCC) interaction miRNA-122-binding site IL-1A rs3783553 hepatitis B virus (HBV) mutations

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Influence of site conditions on seismic design parameters for foundations as determined via nonlinearsite response analysis

Muhammad Tariq A. CHAUDHARY

《结构与土木工程前沿（英文）》 2021年第15卷第1期页码 275-303 doi: 10.1007/s11709-021-0685-0

摘要： Site conditions, including geotechnical properties and the geological setting, influence the near-surface response of strata subjected to seismic excitation. The geotechnical parameters required for the design of foundations include mass density ( ), damping ratio ( ), shear wave velocity ( ), and soil shear modulus ( ). The values of the last three parameters are sensitive to the level of nonlinear strain induced in the strata due to seismic ground motion. In this study, the effect of variations in soil properties, such as plasticity index ( ), effective stress ( ), over consolidation ratio (OCR), impedance contrast ratio ( ) between the bedrock and the overlying strata, and depth of soil strata over bedrock ( ), on seismic design parameters ( , , and ) was investigated for National Earthquake Hazards Reduction Program (NEHRP) site classes C and D, through 1D nonlinear seismic site response analysis. The Morris one-at-a-time (OAT) sensitivity analysis indicated that , , and were significantly influenced by variations in , while affected more than it affected and . However, the influence of on these parameters was less significant. It was also found that variations in soil properties influenced seismic design parameters in soil type D more significantly than in soil type C. Predictive relationships for , , and were derived based on the 1D seismic site response analysis and sensitivity analysis results. The , , and values obtained from the analysis were compared with the corresponding values in NEHRP to determine the similarities and differences between the two sets of values. The need to incorporate and in the metrics for determining , , and for the seismic design of foundations was highlighted.

关键词： site effects 1D seismic site response analysis sensitivity analysis foundations shear wave velocity soil shear modulus

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Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

《医学前沿（英文）》 2022年第16卷第3期页码 378-388 doi: 10.1007/s11684-021-0840-y

摘要： Macrolide and corticosteroid resistance has been reported in patients with Mycoplasma pneumoniae (MP) pneumonia (MPP). MP clearance is difficult to achieve through antibiotic treatment in sensitive patients with severe MPP (SMPP). SMPP in children might progress to airway remodeling and even bronchiolitis/bronchitis obliterans. Therefore, identifying serum biomarkers that indicate MPP progression and exploring new targeted drugs for SMPP treatment require urgency. In this study, serum samples were collected from patients with general MPP (GMPP) and SMPP to conduct proteomics profiling. The Fc fragment of the IgG-binding protein (FCGBP) was identified as the most promising indicator of SMPP. Biological enrichment analysis indicated uncontrolled inflammation in SMPP. ELISA results proved that the FCGBP level in patients with SMPP was substantially higher than that in patients with GMPP. Furthermore, the FCGBP levels showed a decreasing trend in patients with GMPP but the opposite trend in patients with SMPP during disease progression. Connectivity map analyses identified 25 possible targeted drugs for SMPP treatment. Among them, a mechanistic target of rapamycin kinase (mTOR) inhibitor, which is a macrolide compound and a cell proliferation inhibitor, was the most promising candidate for targeting SMPP. To our knowledge, this study was the first proteomics-based characterization of patients with SMPP and GMPP.

关键词： severe Mycoplasma pneumoniae pneumonia children proteomics Fc fragment of the IgG-binding protein mechanistic target of rapamycin kinase inhibitor

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Chloride binding and time-dependent surface chloride content models for fly ash concrete

S. MUTHULINGAM,B. N. RAO

《结构与土木工程前沿（英文）》 2016年第10卷第1期页码 112-120 doi: 10.1007/s11709-015-0322-x

摘要： Corrosion of embedded rebars is a classical deterioration mechanism of reinforced concrete structures exposed to chloride environments. Such environments can be attributed to the presence of seawater, deicing or sea-salts, which have high concentrations of chloride ion. Chloride ingress into concrete, essential for inducing rebar corrosion, is a complex interaction between many physical and chemical processes. The current study proposes two chloride ingress parameter models for fly ash concrete, namely: 1) surface chloride content under tidal exposure condition; and 2) chloride binding. First, inconsistencies in surface chloride content and chloride binding models reported in literature, due to them not being in line with past research studies, are pointed out. Secondly, to avoid such inconsistencies, surface chloride content and chloride binding models for fly ash concrete are proposed based upon the experimental work done by other researchers. It is observed that, proposed models are simple, consistent and in line with past research studies reported in literature.

关键词： binding isotherms chloride ingress concrete fly ash surface chloride content

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MiRNA-451 is a potential biomarker for estrogenicity in mouse uterus

Lingyan HOU, Yun LU, Ying LI, Li LI

《环境科学与工程前沿（英文）》 2014年第8卷第1期页码 99-105 doi: 10.1007/s11783-013-0490-7

摘要： The uterotrophic assay has been commonly used to test environmental estrogens in vivo, however, it is often not sensitive enough sometimes. An alternative way is to evaluate estrogenicity through biomarker genes. MicroRNA (miRNA) is a class of regulatory gene, which has been shown to be a good biomarker for many diseases and toxicological effects in recent years, and some evidences showed that estrogen induced response was partially mediated by miRNAs. In this study, two types of microarrays were used to test the 17β-estradiol (E2) induced miRNA expression profile at different time points in the immature mouse uterus. Statistical analysis showed the aldehyde slide based array had less variation than the amino slide based array, and 11 dysregulated miRNAs were screened out for significant fold change. Real-time PCR was performed to further confirm that 4 out of 7 selected miRNAs, namely miR-451, miR-155, miR-335-5p, and miR-365, are E2 regulated miRNAs in the uterus. The function of the predicted targets of these miRNAs is involved in cell grow control, which is consistent with the main E2 function in the uterus. MiR-451 had similar strong responses to E2 in the uterus of both immature and overiectomized mice, and could be a potential biomarker for estrogenicity in the uterus.

关键词： estrogen microRNA (miRNA) microarray biomarker

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Development and application of an off-site maintenance system in the petrochemical industry

Ruisheng YONG, Yanbing YE, Hanbin LUO, Lieyun DING

《工程管理前沿（英文）》 2017年第4卷第2期页码 111-126 doi: 10.15302/J-FEM-2017027

摘要： On-site maintenance (ONSM) is critical to ensuring the safety of equipment in the petrochemical industry. However, many accidents occur during ONSM processes because of unstable equipment, complicated work environment, and human error. To reduce congestion and exposure to hazards during on-site operations and thereby reduce the probability of accidents, off-site maintenance (OFSM) is proposed based on Energy Release Theory (ERT). The information requirements for OFSM are analyzed. A support system for OFSM, which makes use of visualization, information management, and localization capabilities, is developed. A case study utilizing OFSM and its support system for an oil-loading facility of a petrochemical company is conducted. The application results indicated that implementation of OFSM system can significantly reduce the operation risks and can improve the operation efficiency.

关键词： on-site maintenance petrochemical industry safety off-site maintenance system

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reduces IgE binding ability of allergenic egg white proteins

Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU

《农业科学与工程前沿（英文）》 2018年第5卷第3期页码 373-381 doi: 10.15302/J-FASE-2018210

摘要：

Egg white proteins are one of the major allergens. The objective of this study was to investigate the effect of Aspergillus oryzae cultivation on IgE binding ability of egg white proteins. Effect of A. oryzae on egg white proteins was determined using ninhydrin method, SDS-PAGE, ELISA, fluorescence FITC labeling, MALDI-TOF-MS and LC-MS/MS analysis. Adding mycelium of A. oryzae ATCC 1011 and 16868 substantially reduced the IgE binding ability of acidified egg white after 24 h incubation. The binding capacity of egg white proteins to IgE in plasma from four egg allergy patients was almost completely lost after incubation with mycelium of ATCC 16868. Results from SDS-PAGE, free amino acid analysis, MALDI-TOF-MS and LC-MS/MS indicated that there was no substantial protein degradation during incubation. Therefore, the reduction of IgE binding ability of egg white proteins during A. oryzae treatment was probably due to a loss of ~1700 Da mass including a fragment of the ovomucoid N terminus.

关键词： Aspergillus oryzae egg allergy egg white proteins IgE-binding ability ovomucoid

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Quantitative characterization of Cu binding potential of dissolved organic matter (DOM) in sediment from

Yuan ZHANG,Yan ZHANG,Tao YU

《环境科学与工程前沿（英文）》 2014年第8卷第5期页码 666-674 doi: 10.1007/s11783-013-0608-y

摘要： Dissolved organic matter (DOM) plays an important role in heavy metal speciation and distribution in the aquatic environment especially for eutrophic lakes which have higher DOM concentration. Taihu Lake is the third largest freshwater and a high eutrophic lake in the downstream of the Yangtze River, China. In the lake, frequent breakout of algae blooms greatly increased the concentration of different organic matters in the lake sediment. In this study, sediment samples were collected from various part of Taihu Lake to explore the spatial difference in the binding potential of DOM with Cu. The titration experiment was adopted to quantitatively characterize the interaction between Cu(II) and DOM extracted from Taihu Lake sediments using ion selective electrode (ISE) and fluorescence quenching technology. The ISE results showed that the exogenous DOM had higher binding ability than endogenous DOM, and DOM derived from aquatic macrophytes had a higher binding ability than that derived from algae. The fluorescence quenching results indicated that humic substances played a key role in the complexation between DOM and Cu(II) in the lake. However, because of the frequent breakout of algae blooms, protein-like matters are also main component like humic matters in Taihu Lake. Therefore, the metals bound by protein-like substances should be caused concern as protein-like substances in DOM were unstable and they will release bound metal when decomposed.

关键词： binding ability dissolved organic matters fluorescence quenching complex capacity Taihu Lake

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Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block

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《医学前沿（英文）》 2016年第10卷第4期页码 420-429 doi: 10.1007/s11684-016-0478-3

摘要：

Inappropriate cell proliferation during oncogenesis is often accompanied by inactivation of components involved in the cell cycle machinery. Here, we report that cyclin-dependent kinase inhibitor 2C (CDKN2C) as a member of the cyclin-dependent kinase inhibitors is a target of the PML/RARα oncofusion protein in leukemogenesis of acute promyelocytic leukemia (APL). We found that CDKN2C was markedly downregulated in APL blasts compared with normal promyelocytes. Chromatin immunoprecipitation combined with quantitative polymerase chain reaction demonstrated that PML/RARα directly bound to the CDKN2C promoter in the APL patient-derived cell line NB4. Luciferase assays indicated that PML/RARα inhibited the CDKN2C promoter activity in a dose-dependent manner. Furthermore, all-trans retinoic acid treatment induced CDKN2C expression by releasing the PML/RARα binding on chromatin in NB4 cells. Functional studies showed that ectopic expression of CDKN2C induced a cell cycle arrest at the G₀/G₁ phase and a partial differentiation in NB4 cells. Finally, the transcriptional regulation of CDKN2C was validated in primary APL patient samples. Collectively, this study highlights the importance of CDKN2C inactivation in the abnormal cell cycle progression and differentiation block of APL cells and may provide new insights into the study of pathogenesis and targeted therapy of APL.

关键词： CDKN2C acute promyelocytic leukemia cell cycle arrest differentiation

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促进肝癌致癌活性和细胞代谢的新型介质——miR-516a-3p

芮韬, 张学优, 冯时, 黄海涛, 詹少伟, 谢海洋, 周琳, 郑树森, 凌琪

《工程（英文）》 2022年第16卷第9期页码 162-175 doi: 10.1016/j.eng.2021.07.020

摘要：根据先前的研究，19 号染色体miRNA簇（C19MC）与肝癌患者的肿瘤高负荷和不良预后相关。目前的研究旨在探讨miR-516a-3p 在HCC 中的作用。miR-516a-3p 是一种由C19MC 4 个致癌前体miRNA（即mir-516a-1、mir-516a-2、mir-516b-1 和mir-516b-2）所共同剪接而成的相同的成熟体miRNA。

关键词：肝细胞肝癌 miRNA簇外泌体多组学

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An integrated approach to site-specific management zone delineation

Yuxin MIAO, David J. MULLA, Pierre C. ROBERT

《农业科学与工程前沿（英文）》 2018年第5卷第4期页码 432-441 doi: 10.15302/J-FASE-2018230

摘要：

Dividing fields into a few relatively homogeneous management zones (MZ) is a practical and cost-effective approach to precision agriculture. There are three basic approaches to MZ delineation using soil and/or landscape properties, yield information, and both sources of information. The objective of this study is to propose an integrated approach to delineating site-specific MZ using relative elevation, organic matter, slope, electrical conductivity, yield spatial trend map, and yield temporal stability map (ROSE-YSTTS) and evaluate it against two other approaches using only soil and landscape information (ROSE) or clustering multiple year yield maps (CMYYM). The study was carried out on two no-till corn-soybean rotation fields in eastern Illinois, USA. Two years of nitrogen (N) rate experiments were conducted in Field B to evaluate the delineated MZs for site-specific N management. It was found that in general the ROSE approach was least effective in accounting for crop yield variability (8.0%–9.8%), while the CMYYM approach was least effective in accounting for soil and landscape (8.9%–38.1%), and soil nutrient and pH variability (9.4%–14.5%). The integrated ROSE-YSTTS approach was reasonably effective in accounting for the three sources of variability (38.6%–48.9%, 16.1%–17.3% and 13.2%–18.7% for soil and landscape, nutrient and pH, and yield variability, respectively), being either the best or second best approach. It was also found that the ROSE-YSTTS approach was effective in defining zones with high, medium and low economically optimum N rates. It is concluded that the integrated ROSE-YSTTS approach combining soil, landscape and yield spatial-temporal variability information can overcome the weaknesses of approaches using only soil, landscape or yield information, and is more robust for MZ delineation. It also has the potential for site-specific N management for improved economic returns. More studies are needed to further evaluate their appropriateness for precision N and crop management.

关键词： economically optimum nitrogen rate fuzzy cluster analysis precision nitrogen management site-specific management soil landscape property yield map

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Inflammation and liver tumorigenesis

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《医学前沿（英文）》 2013年第7卷第2期页码 242-254 doi: 10.1007/s11684-013-0256-4

摘要：

Inflammation has been considered as one of the hallmarks of cancer, and chronic hepatitis is a major cause of liver cancer. This review will focus on the pathogenic role of inflammation in hepatocarcinogenesis and will discuss recent advances in understanding the chronic hepatitis-liver cancer link based on hot spots in liver cancer research, including cellular interaction, cytokines, microRNA and stem cells. All of these mechanisms should be taken into consideration because they are crucial for the development of more efficacious therapeutic strategies for preventing and treating human chronic hepatitis and hepatocellular carcinoma.

关键词： hepatocellular carcinoma hepatitis cytokine stem cell miRNA

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Site-directed mutagenesis of long QT syndrome KCNQ1 gene

LI Wei, WANG Bin, XU Qiumei, KE Qinmei, YANG Junguo, DU Rong, TIAN Li, WANG Qing

《医学前沿（英文）》 2008年第2卷第1期页码 100-104 doi: 10.1007/s11684-008-0018-x

摘要： To construct a polymerase chain reaction (PCR) site-directed mutagenesis of the long QT syndrome KCNQ1 gene , two sets of primers were designed according to the sequence of KCNQ1 cDNA and a mismatch was introduced into primers. Mutagenesis was performed in a two-step PCR. The amplified fragments from the third PCR which contained the mutation site were sub-cloned into the T-vector pCR2.1. Then, the fragments containing the mutation site was obtained from pCR2.1 using restriction enzymes digestion and inserted into the same restriction site of pIRES-EGFP-KCNQ1. The sequencing analysis shows that the mutation site was correct. Mutation from A to G in site 983 of KCNQ1 cDNA was found. Using the Effectene transfection reagent, pIRES-EGFP-KCNQ1 (G983A) was transfected into HEK cells successfully. These results may shed light on further functional study of KCNQ1 gene.

关键词： restriction digestion syndrome sequence site-directed mutagenesis

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Construction Technology of Off-Site Precast Concrete Buildings

Ting Gong,Jian Yang,Hao Hu,Feng Xu

《工程管理前沿（英文）》 2015年第2卷第2期页码 122-124 doi: 10.15302/J-FEM-2015039

摘要： The industrialization of building construction is gradually becoming a new choice of construction technology driven by some great advantages such as high efficiency in construction, low energy consumption and environmental friendliness. Construction management plays an important part to ensure the quality of projects. A comparison between traditional on-site and modern off-site construction methods has been conducted. The streamlining of the process including the fabrication, transportation, stacking and assembling of precast components has also been analyzed with a discussion on the BIM application in the process.

关键词： off-site construction precast concrete modular construction construction management building information model (BIM)

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Diffusive gradients in thin films using molecularly imprinted polymer binding gels for in situ measurements

Ying Cui, Feng Tan, Yan Wang, Suyu Ren, Jingwen Chen

《环境科学与工程前沿（英文）》 2020年第14卷第6期 doi: 10.1007/s11783-020-1290-5

摘要： Abstract • Selective molecularly imprinted polymer (MIP) binding gel was prepared. • MIP-DGT showed excellent uptake performance for antibiotics. • In situ measurement of antibiotics in wastewaters via MIP-DGT was developed. • The MIP-DGT method was robust, reliable, and highly sensitive. Urban wastewater is one of main sources for the introduction of antibiotics into the environment. Monitoring the concentrations of antibiotics in wastewater is necessary for estimating the amount of antibiotics discharged into the environment through urban wastewater treatment systems. In this study, we report a novel diffusive gradient in thin films (DGT) method based on molecularly imprinted polymers (MIPs) for in situ measurement of two typical antibiotics, fluoroquinolones (FQs) and sulfonamides (SAs) in urban wastewater. MIPs show specific adsorption toward their templates and their structural analogs, resulting in the selective uptake of the two target antibiotics during MIP-DGT deployment. The uptake performance of the MIP-DGTs was evaluated in the laboratory and was relatively independent of solution pH (4.0–9.0), ionic strength (1–750 mmol/L), and dissolved organic matter (DOM, 0–20 mg/L). MIP-DGT samplers were tested in the effluent of an urban wastewater treatment plant for field trials, where three SA (sulfamethoxazole, sulfapyridine, and trimethoprim) and one FQ (ofloxacin) antibiotics were detected, with concentrations ranging from 25.50 to 117.58 ng/L, which are consistent with the results measured by grab sampling. The total removal efficiency of the antibiotics was 80.1% by the treatment plant. This study demonstrates that MIP-DGT is an effective tool for in situ monitoring of trace antibiotics in complex urban wastewaters.

关键词： Diffusive gradients in thin films Molecularly imprinted polymers Selective uptake Antibiotics Passive sampling